Phenotypic methods for detection of AmpC –lactamase in Klebsiella species

Rakesh Prasad Sah; Dr. A.D. Urhekar; Rakesh Kumar Mukhia; B. L. Chaudhary

Volume : V, Issue : V, May - 2016

Phenotypic methods for detection of AmpC –lactamase in Klebsiella species

Rakesh Prasad Sah, Dr. A. D. Urhekar, Rakesh Kumar Mukhia, B. L. Chaudhary

Abstract :

Objective: To find incidence of AmpC β–lactamase in Klebsiella species and comparison of two methods, combined disc diffusion test (CDDT) & Modified three dimensional test (M3DT). Methods: All the cefoxitin resistant isolates of Klebsiella species were subjected to determine the AmpC β–lactamase by combined disc diffusion test (Cefoxitin + Phenylboronic acid) & Modified three dimensional test (Enzyme extract method). Result: AmpC β–lactamase was detected in 44 & 55 of the 200 isolates of Klebsiella species by combined disc diffusion test (CDDT) and Modified three dimensional test (M3DT) respectively. Conclusion: AmpC β–lactamase as a drug resistance mechanism was found in 22% strains by CDDT and 27.5% strains by M3DT method. Hence M3DT method is advised to study AmpC β–lactamase in Klebsiella species.