STUDY OF MicroRNA EXPRESSION AS A MOLECULAR BIOMARKER IN CHILDHOOD ACUTE LYMPHOBLASTIC LEUKEMIA

Magdy Mamdouh El Bordiny; Mostafa Ahmed Said Salama; Amani Fouad Sorour; Abeer Shawky El Hadidi; Salma Alaa El Din Mostafa Imbaby

Volume : IV, Issue : XI, November - 2015

STUDY OF MicroRNA EXPRESSION AS A MOLECULAR BIOMARKER IN CHILDHOOD ACUTE LYMPHOBLASTIC LEUKEMIA

Magdy Mamdouh El Bordiny, Mostafa Ahmed Said Salama, Amani Fouad Sorour, Abeer Shawky El Hadidi, Salma Alaa El Din Mostafa Imbaby

Abstract :

<p>&nbsp;Acute lymphoblastic leukemia (ALL) is a heterogeneous group of leukemias that results from the proliferation of lymphoblasts that originates from the lymphocyte progenitor cell of the bone marrow or the thymus. It includes many subtypes that show different clinical behaviors and need different therapy schemes. Many studies in the last few years have shown that differential expression of candidate miRNAs in normal compared to transformed cell provides important insights into the pathogenesis of cancer including leukemia. We aimed to evaluate the expression profile of the microRNAs associated with childhood ALL: miR&ndash;100&amp; miR&ndash;196b and their association with biological/prognostic features in 40 consecutive samples of newly diagnosed childhood ALL by quantitative real&ndash;time PCR. FISH was done for only selected ALL cases for t(12;21) and MLL rearrangement, when required.Both miRNAs analyzed (miRNAs 100 and 196b) were significantly underexpressed in ALL patients relative to control. There was an increased incidence of T&ndash;ALL in cases showing miR196b expression. Cases showing increased miR&ndash;196b expression were significantly overexpressed in CNS affected patients.</p>