IJSR International Journal of Scientific Research 2277-8179 Indian Society for Health and Advanced Research ijsr-5-10-010 Original Research Paper “Study of gibberellic acid production by submerged fermentation using fusarium moniliforme, Sheldon” PanchalRakeshkumar R.Dr. 1 Associate Professor and Head, Dept. of Microbiology, M.B. Patel Science College, Anand – 388001 10 2016 5 10 34 37 Abstract

Gibberellic acid production using Fusarium moniliforme, isolated from wilted sugarcane plant has been investigated by submerged fermentation (SMF). The gibberellic acid production of 195 mgm/ml was obtained on commercial wheat bran extract medium in 250 ml flasks after 168 hrs of incubation. The gibberellic acid production rate was about 0.6 to 0.9 mgm/ml/hr during 96 to 168 hrs. Different carbon sources namely sucrose, lactose, maltose, soluble starch, glycerol, wheat flour and maize flour were tested as an additional carbon source along with CWB extract at the concentration of 2.5% w/w or v/w base to observe its effects on gibberellic acid production. Soluble starch has been proved the best additional carbon source for gibberellic acid production, which yielded 1720 mgm/ml of gibberellic acid after 168 hrs of incubation. Similarly, various nitrogen sources namely NH4Cl, NH4NO3, (NH4) 2SO4, (NH4) MoO4 and Urea were tested as an additional nitrogen source at the concentration of 7.0 mg % level in CWB extract. Urea was proved as the best nitrogen source which yielded 405 mgm/ml of gibberellic acid after 168 hrs of incubation. We have observed about 7.5-fold and 3.5-fold increase in gibberellic acid production upon addition of soluble starch and urea respectively, in CWB extract using Fusarium moniliforme.

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