Extended–spectrum beta–lactamases (ESBLs) are enzymes secreted by some bacteria which make some antibiotics ineffective. Klebsiella pneumoniae and Escherichia coli remain the major ESBL–producing organisms isolated worldwide. This prospective study was conducted in the Department of Microbiology at DM WIMS Medical College, Wayanad. A total of 234 isolates were obtained from clinical samples processed from May to August 2016. All the gram negative rods were screened and tested for ESBL production. ESBL was detected in 19 strains. Out of these 19 ESBL producers 11(57.9%) were Klebsiella followed by E.coli 4 (21%), Acinetobacter 3(15.78%), Pseudomonas 1(5.26%).Routine detection of ESBL–producing microorganisms should be done in each laboratory using the standard detection methods so as to control the spread of these infections and also to implement proper therapeutic strategies.