An imbalance between reactive oxygen species production and antioxidant scavenging has been implicated in diabetes. Reports indicate that several complications of diabetes mellitus are associated with increased activity of free radicals and accumulation of lipid peroxidation products. The correlation of Cannabis user groups with duration of one–month, one–year and five year (Both diabetic and non–diabetic groups) with serum Fasting Blood Glucose level and serum Malondialdehyde (MDA) level. The present study was conducted on 105 male–subjects aged between 25 and 65 years. This study was conducted at Department of Biochemistry, Sardar Patel Medical College, Bikaner, India. Fasting blood samples were obtained from 55 diabetic male subjects and 50 healthy control subjects. Blood samples were withdrawn who were taking 5 to 10 gm of cannabis per day and analyzed for Fasting Blood Glucose and serum Malondialdehyde (MDA) by standard technique using UV–Spectrophotometer. Fasting Blood Glucose and serum Malondialdehyde (MDA) were measured and the results were compared with those of controls. Fasting Blood Glucose and MDA levels were found to be significantly lower in diabetic and non–diabetic subjects using cannabis 5 to 10 gm per day for the last one month, one year and five years when compare with normal control and diabetic control subjects(p<0.01). Study shows a significant decreased in blood sugar and MDA level recorded diabetic patients and non diabetic groups using cannabis for the last one year and five year as well as in the whole group as compared to that of normal control. It might be possible that either cannabis may enhanced the insulin release or increase the penetration of sugar into the cells and might be due to antioxidant activity of cannabis.