Background: Throughout the world multi–drug resistant nosocomial infections are one of the leading causes of deaths and morbidity amongst hospitalized patients. It is critical to understand microbiology of these infections in order to create appropriate strategies to reduce this risk. Objectives: Objective of the study was to determine the pattern of pathogens involved and their antibiotic sensitivity and resistance isolated from different ICU patients of a tertiary care hospital in Central part of India. Materials and Methods: The study was conducted in the Intensive Care Unit of Pt JNM Medical College, a tertiary care hospital in Ripur during January, 2016 to June 2016. Patients admitted in ICU of the hospital who were clinically suspected of having acquired any infection after 48 hours of admission to the ICU were included. Depending on the clinical suspicion laboratory samples were collected from the patients. Samples were subjected to the testing and antibiotic sensitivity. Results and Observations: The commonest organisms isolated from all samples in ICU were E.coli, Klebsiella spp. and Staph aureus. A total 400 patient’s samples were analyzed which included blood 275 (68.75%), swab 34 (8.50%), body fluids 30 (7.50%), urine 36 (9.0%), pus 15 (3.75%), sputum 10 (2.50%). Total 140 (35.0%) samples were positive for growth of the organisms. Penicillin derivatives (Pipercillin/Tazobactam) and carbapenem eg. Imipenem are the most sensitive antibiotics covering all the organisms isolated in our study. Conclusion: Nosocomial infections and antimicrobial resistance in the ICUs is a major deterrent to patient’s outcome, increasing duration of patient stay as well as expense. Reduction of the same is both challenge and goal of all intensive care units around world. The increasing trend of resistance to b–lactams is posing a great problem. So for proper management of critically ill patients and patients undergoing various operative procedures and other medical interventions, hospital antibiotic policies need frequent revisions.