Thyroid hormones regulate metabolism. According to different studies worldwide it has been seen that in hypothyroid subjects, LDH and CPK levels were markedly elevated due to involvement of hepatocytes. The aims of this study were to measure the level of serum creatine kinase (CK) and lactate deydrogenase (LDH) in hypothyroid patients, and to compare the level of CK & LDH levels in hypothyroid & euthyroid individuals.92 patients coming to Dept. of Biochemistry & Dept. of Endocrinology in NRS Medical college were tested for CPK,LDH,FT4 & TSH. In hypothyroid individuals mean CPK level was  185.4012+–61.73301IU/L & mean LDH level was 270.8472+–44.05418 IU/L,both the values were found to be statistically significantly higher than the euthyroid group.(p value < 0.001). Limitation of this study was small sample size etc.