FNA of the testis is a minimally traumatic diagnostic method for evaluation of all the testicular lesions. OBJECTIVE – To study the diagnostic utility of FNA in non–neoplastic and neoplastic lesions of testis and scrotum and to assess the potential of FNA by replacing testicular biopsy in assessment of spermatogenesis in male infertility. MATERIAL AND METHODS– In a retrospective study of 2 years and 9 months, a total of 132 cases of testicular and extratesticular FNA were retrieved. RESULTS– A total of 132 cases of testicular and extratesticular palpable lesions were included in the study. Out of 132 cases, 96 aspirates were done for assessment of infertility comprising 72.72% of the total cases which was followed by non–inflammatory, inflammatory, neoplastic and unsatisfactory cases constituting 12.12%, 8.33%, 3.78% and 3.03% respectively. CONCLUSION– FNA of testis and scrotum is a minimally invasive, rapid and reliable outpatient procedure providing adequate sample from the representative areas without any risk of complication.