Haemoglobin is an oxygen binding chromo protein in the red blood corpuscles. International standard of haemoglobin estimation is cyanide method (HiCN). This method is known to be hazardous to the environment and harmful for the laboratory personnel as it contains KCN    Aims and Objectives:  To compare and study the haemoglobin conc. which were estimated by cyanide and non–cyanide methods. To estimate the advantages of the cyanide free methods over the cyanide methods. To assess whether these methods were better as compared to the haemoglobin cyanide method, for  laboratory investigations. Materials and Methods: 1000 samples were tested for haemoglobin were done by the cyanmeth haemoglobin method and modified alkaline haematin method which uses cetrimide were compared using Pearson’s correlation coefficient. Results: By the modified alkali denaturation method, haemoglobin concentration, were similar, to those, which were obtained by the conventional cyanmethaemoglobin  method Conclusion: The modified alkali denaturation method method was as accurate as the cyanmethaemoglobin method. It was economical. The toxic effects of cyanide can be prevented by using this method commercially.