Haemoglobin is an oxygen binding chromo protein in the red blood corpuscles.The international standard of estimation by the cyanide method (HiCN). This method is known to be hazardous to the environment and harmful to the laboratory personnel as it contains KCN    Aims and Objectives: Compare and study the haemoglobin conc. which were estimated by cyanide and non–cyanide methods. To estimate the advantages of the cyanide free methods over the cyanide methods. To assess whether these methods were better as compared to the haemoglobincyanide method, for  laboratory investigations. Materials and Methods: 1000 samples were tested for haemoglobin were done by the cyanmeth method and Sodium Lauryl Sulphate method (SLS)  Was compared using Pearson’s correlation coefficient. Results: The haemoglobin concentrations which were estimated by the Sodium Lauryl Sulphate method were similar to those which were obtained by the conventional cyanmethaemoglobin  method Conclusion: The SLS method was as accurate as the cyanmethaemoglobin method. It was economical. The toxic effects of cyanide can be prevented by using this method commercially.