Enterococci  have evolved from being commensal gut flora of  little clinical significance to becoming an increasingly important cause of nosocomial infection in the last decade .The present study was undertaken to identify the species prevalence , detect vancomycin resistance among enterococci and to determine antimicrobial resistance profile.A total of 134  isolates obtained from various clinical samples were  speciated by Facklam and Collins scheme.Resistance to glycopeptides were determined by agar dilution and  Minimum Inhibitory Concentration(MIC) was determined by E test.Out of  134 isolates studied, 127 (94.78%) were  Enterococcus faecalis and 7(5.22%) were Enterococcus faecium. . Out of  107  Enterococcus faecalis studied from urine sample,  two  isolates were  with Minimum Inhibitory Concentration of   ≥ 32 μg/ml  for both  vancomycin  and teicoplanin by Agar dilution method  and E test  .Among the Enterococcus faecium isolates ,5 isolates  from urine were with MIC range of  0.5 to 2 μg/ml for  vancomycin    by E  test.