Uropathogenic Escherichia coli, the most frequent pathogens in acute urinary tract infections, take advantage of assortment of virulence properties. Bacterial adherence to and colonization of the urinary tract by UPEC strains are mediated by several types of virulence factors which need to be evaluated. Total 125 E. coli isolates were screened for different virulence markers viz Serum Resistance (SR), Adherence to epithelial cells (AD), Haemagglutination (HA) and haemolysin production (HL). Their antimicrobial susceptibility was also studied. Out of 125 isolates of E.coli; 35 were from Male, 78 from Female patients and 12 were from Children. SR was commonest as seen in 98 (72%) cases followed by 79 (63.2%) positive for AD while HA and HL production was seen in only 28 (22.4%) and 24 (19.2%) respectively. The virulence markers were present either alone or simultaneously with other virulence markers with varying frequencies. The antimicrobial susceptibility profile showed the strains to be multidrug resistant. As many as 89.6% UPEC strains display virulence markers. The Serum Resistance and adhesion appears to be the most consistent virulence markers and play a major role in their pathogenicity. Multiple virulence markers may appear simultaneously. These strains are generally multidrug resistant.