Acinetobacter species has been increasingly reported as the cause of nosocomial infections and possess a serious threat to the health care system because of its multi–drug resistance. My aim was to determine the Frequency, and antibiotic resistance pattern of Acinetobacter species from various clinical samples. A total of 850 patients clinical samples were included in present study, out of which 40(4.7%) showed growth of Acinetobacter species. Acinetobacter species were isolated highest from blood 20(50%), followed by pus 1(27.5%), CSF 4(10%), urine 2(5%), sputum 2(5%), plural fluid 1(2.5%) and tracheal aspirate 0 (0%). The study will help to implement better infection control strategies and improve the knowledge of antibiotic resistance patterns of Acinetobacter species in our region.