INTRODUCTION: Transfusion of blood and blood products is life saving in various medical and surgical conditions which carries 1% chance of transfusion related complications including transfusion transmisable infections (TTIs) like HIV, Hepatitis B, Hepatitis C, Syphilis and Malaria. AIM: To find the seroprevalence of TTIs in voluntary blood donors in a  medicl  college hospital blood bank,Tamilnadu. MATERIAL AND METHODS: This was a retrospective hospital records based 6 year study, included 60422 voluntary blood donors from 2009  to  2015 and screened for TTIs.  RESULTS: 729 cases of TTIs constituting (1.2%) were detected, of which 54(0.001%) were seroreactive for HIV,  636(0.01%) for Hepatitis B, 33(0.0005%) for Hepatitis C and  6(0.0001%) for  Syphilis. Malaria(0%) was detected in none of the donors. CONCLUSION: In the present study,  the  seroprevalence of  HIV,Hepatitis B,Hepatitis C,  Syphilis and Malaria  is lower than  other studies from various parts of the country.  A decreasing  trend  in  the  seroprevalence  of  TTIs  is  noted  in  this  region  of  Tamilnadu, India.