Introduction World health organization estimated in 2004 that 12.2% of worldwide deaths were from ischemic heart disease which is leading cause of death in myocardial infarction. National health and nutritional examination survey, 1988–1994, first time reported a significant positive association in iron storage and heart disease risk. The study was done to find out the relation of serum ferritin with MI. Materials methods A total of 50 patients who were confirmed of having MI on the basis of lipid profile, CKMB, SGOT, SGPT, Trop I, ECG and Chest X Ray were included in the study. Serum ferritin level was estimated in study group on day 1 and day 6 of MI attack while only once in control. Serum ferritin was estimated by enzyme linked fluorescent assay on Mini Vidas auto analyzer from Biomerieux. Results The present study conducted on MI patients of western U.P. showed that serum ferritin levels on day 1 of MI was 60.64 ±19.07 and day 6 of MI was 224.4 ± 96.86 ng/ml which was significantly higher on day 6. The value of serum ferritin in controls was 75.59 ± 35.89 ng/ml. Conclusion Serum ferritin levels were found to be lower and statistically significant in MI patients on day 1 of disease when compared with the values of control group.