Electrolyte disturbance reported in PTB, HIV and HIV–PTB patients. We conducted prospective study in 172 patients, to evaluate & compare electrolyte imbalance in PTB, HIV & HIV–PTB. All patients were screened for PTB, HIV & levels of serum Sodium, Potassium & chloride Levels Using Standard methods. Study showed Lower mean values of sodium were seen in HIV–PTB, PTB, HIV Vs Control. Lower mean values of potassium in PTB Vs HIV–PTB, HIV. Higher mean values of potassium in HIV, Chlorides in HIV–PTB was found compared to controls. Lower mean values of chloride were found in PTB Vs Control, HIV–PTB. This study showed Hyponatremia, Hypokalemia, hypochloremia characterised some of electrolyte imbalance in PTB patients. Hyperkalemia in HIV patients & Hyperchloremia in HIV–PTB were seen. Strict Monitoring of serum electrolytes in PTB, HIV–PTB, and HIV is recommended, as early detection and treatment will enhance the quality of life of patients.