INTRODUCTION: Diabetes Mellitus is polygenic and multifactorial disease , since in addition to genetic susceptibility, environmental factors such as obesity, nutrition, and physical activity modulate the phenotype. Type 2 Diabetes Mellitus has a strong genetic component and autonomic nervous system dysregulation is implicated in the pathogenesis of obesity and type 2 Diabetes Mellitus. The present study determines the changes in cardiac autonomic functions by evaluating the effect of deep breathing on heart rate in healthy nondiabetic offsprings of type 2 diabetes patients and to compare it with age and sex matched controls. METHODOLOGY: This study of Cardiovascular Autonomic  Functions was carried out in 50  healthy offsprings of Type 2 Diabetic Parents (Case group) and 50 healthy offsprings of Nondiabetic Parents (Control group) in the age range of 18 – 25 years randomly selected among  MBBS students of J.L.N. Medical College Ajmer. Statistical Analysis is done by student t–test. Probability P value <0.05 was considered statistically significant. RESULT: : In offsprings of type 2 diabetic patients  significantly higher basal heart rate (beats/min) (P< 0.001) and  significantly lower deep breathing E/I ratio (P <0.001) were observed  as compared to control group. CONCLUSION: The study reveals shift in sympathovagal balance was due to vagal withdrawal   in offspring of type 2 Diabetes Mellitus patients. Risk of future diabetes is associated with this autonomic dysfunction .Early detection can be utilized to prevent future cardiovascular risk in subjects by  non pharmacological means as exercise and yoga.