Aim: Aim of this study was to evaluate the efficacy of autogenous dentin graft in bone defect of rabbit. Material and method: 6 rabbit were selected for this study and divided into 3 groups. Group 1 and 2 are experimental group, group 3 control group. Mechanically bony lesions were created in rabbit mandible and tibia unilaterally and graft material is placed and defect is sutured. Autogenous dentine graft used in this study. Animals were sacrificed for histological observation of bone defect at 4th week and 6th week respectively Result: group 1 and 2 shows bone formation in bone defect surrounding to dentine graft while as in group 3 (control grp) defect is not completely filled with bone. Discussion: Many of the allografts and Xenografts on the market are not sterile since the sterilization process ruins the organic collagen aspect of the graft that many manufacturers want to keep intact. Autogenous dentine graft said to be gold standard and shows promising results and have many advantages Conclusion: Within the limitations of this study, autogenous dentin graft material made from extracted teeth may have potential as a bone graft material for bone formation.