Acrylic resin is susceptible to change in surface properties when subjected  to different mechanical and chemical denture cleansing methods, which may result in a rough surface that makes the prosthesis more susceptible to plaque accumulation. Objective :To evaluate the effect of various denture cleansing methods on the surface roughness of denture teeth and denture base. Method: 120 denture teeth and denture base specimens were divided into 4 groups (n=30): distilled water(control), alkaline hypochlorite, alkaline peroxide and toothbrush with soap and tap water. The samples were stored in their respective solutions for 8 hours , over a period of 6months.Surface roughness of the samples was evaluated at varying intervals using a profilometer. Results : Significant differences (p<0.05) were found in surface roughness values of denture base and denture teeth after 6 months. Conclusion: The time period and the type of denture cleansing method  had a significant influence on the surface roughness of the specimens.