Introduction Liver is composed of series of segments that combine to form sectors each of which receives a portal pedicle. This portal pedicle divides into right and left which enters the respective lobes and subdivide again to form the segmental branches thus dividing the sectors into 8 segments. In the past decades, lobotomies were regularly performed, where a large amount of liver tissue was sacrificed for a small tumor present and the results were poor. With more conservative surgeries and post–operative treatment procedures, the segmental pattern of the liver becomes important. Aim To study the segmental anatomy of liver by using manual, radiological and ultrasonic method. Material and methods This study of segmental anatomy of liver was conducted in 100 specimens in the Cadaveric manual dissection method, Radiological method, and Ultrasound method Results In this study the three major hepatic veins, the right hepatic vein, left hepatic vein and the middle hepatic vein were observed in all the specimen. Primarily, the middle hepatic vein join with the left hepatic vein which form a common trunk before draining into the inferior vena cava. Secondly, Portal vein had bifurcation pattern of division into right and left portal vein and had segmental division to all the segments of the liver. Discussion Knowledge of the segmental anatomy very much used for the postoperative treatment mainly the patients posted for lobotomies of liver or segmental surgeries need an intensive nursing care to avoid post–operative complications such as pulmonary complications, deep vein thrombosis, incision hernia, post–operative incision pain and delayed healing of surgical sutures. The variations at this segmental level should be kept in mind to avoid disastrous results. So, the need for more limited resection of the liver has prompted the researchers to look closer into the segmental anatomy of the liver and find more about this organ as well as the anomalies that can spring surprises.