Background: Evidence based practice (EBP) is a systematic approach to clinical problem solving which allows the integration of the best available research evidence with clinical expertise and patient values. Objective: To assess and compare the Knowledge, Attitude and perceived barriers towards EBP among medical academicians and private practitioners in Pune city. Methodology: A cross–sectional study was conducted amongst medical– academicians (n=150) and private practitioners (n=150) in Pune city, using self–administered, pretested, validated, close ended, structured questionnaire. Data was analysed using descriptive statistics, Chi–square test and unpaired t–test. Results: Among the medical academicians and private practitioners, 59 (39.3%) and 77 (51.3%) respectively showed poor knowledge whereas 81 (60.7%) and 73 (49.7%) respectively showed fair knowledge regarding EBP demonstrating a statistically significant difference between their mean knowledge score, (p–0.014). The attitude of the medical academicians 93 (62.0%) and private practitioners 118 (78.6%) towards EBP was positive. Barriers to its use included lack of available time and lack of application of evidence in patients. Conclusion: It was found that significant proportion of professionals had inadequate knowledge of EBP but showed a positive attitude towards it, therefore, a formal training and reinforcement is required.