OBJECTIVE :  To determine the prevalence of Methicillin Resistant Staphylococcus aureus (MRSA) in our institution.   METHODS :  A 5 Month period prevalence study of all specimens. Excluded areas were OPD patients. Our facility is a referral/teaching hospital in Govt sector in Western Maharashtra, Kolhapur. A total of 1340 Specimens were received from various specialties across hospital. Cefoxitin disc diffusion was used to identify methicillin resistance amongst isolated Staphylococci.   RESULTS :  The total number of specimens were 1340 and of those 54 % were gram positive isolates. In these,10 % were Enterococcus, 8 % Streptococcus, and rest 82 % Staphylococci. Out of Staphylococci – 10.5% were methicillin resistant.   CONCLUSION :  The overall prevalence of gram–positive infections is high in specimens we received in our hospital. Though prevalence of MRSA remains concordant with many of the published Indian studies. Limitations being not all patients followed for clinical correlation.