Background: The present study was carried out to evaluate the different techniques for extraction and cleaning of bone specimen from human cadavers. Material and methods: Skull, long bones, and other bones from embalmed preserved cadaver were obtained and cleaned. In present study, we applied 4 different methods for preparing the bone specimen. Results: In present study, we observed that boiling followed by burial of embalmed specimen is ideal procedure for extracting bone specimen of skull and long bones. Conclusions: Preparation of bone specimen from embalmed cadaver is a time saving technique for many medical institutions and self–prepared specimens are of good quality.