The purpose of this study was to comparatively evaluate the effect of two denture cleansers on the stain removal and flexural strength of denture base acrylic resins. 300 samples of heat cure acrylic resin were fabricated and were first inserted into the stain solutions and were finally inserted into two denture cleansers – Fittydent and Clinsodent for 8 hours with distilled water as control. The samples were tested for colour evaluation and flexural strength after 1st, 2nd and 3rd month on uv/vis spectrophotometer and universal testing machine respectively. There was evident color change as well as there was decrease in the flexural strength in all the samples. The study concluded that denture cleansers cause a decrease in the flexural strength of the heat cure acrylic resins as well they cause a change in their color over a longer period of use.