Introduction: H1N1 influenza, a virus having symptoms though not specific of the infection initially is life threatening. Dentist come across many patients including those with undiagnosed cases of the infection leading to spread of virus unknowingly which highlights importance of prevention.  Methodology: A cross–sectional questionnaire based study was conducted amongst 270 dental students and interns studying in a dental college in Pune to assess of the knowledge, attitude and practice towards preventive measures of H1N1 influenza. Chi–square test of proportion and Analysis of Variance (ANOVA) was used. Results: Out of 247 participants 129 (52.22%) participants had poor knowledge regarding H1N1 influenza infection and 166 (67.2%) participants had good attitude. There was statistically significant difference in knowledge score between three groups. (p < 0.05). Conclusion: Majority of the participants had good attitude but there was lack of adequate knowledge.