In the present study 200 urine samples were collected from UTI patients of various Hospital, Thanjavur, Tamil Nadu. The urine sample was stored on specific aseptic containers. From the samples to isolate bacterial species were isolated using UTI agar and MacConkey agar medium. The bacteria isolated were identified with the help of culture morphological and biochemical characteristics. The bacterial isolates were confirmed as Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, and Staphylococcus aureus.   The sensitivity tests were performed to detect the sensitivity of organisms against bacterial antibiotics. In bacteria, the maximum inhibition was observed in antibiotics Meropenem and Nitrofuration. Antibacterial with anticipated effectiveness in patients with chronic renal insufficiency is selected Meropenem and Nitrofuration. Other antibiotics should not be used because of low antibacterial activity against all isolated bacteria. The Staphylococcus aureus have more resistance to all used antibiotics.