Premature ovarian failure (POF) is a common cause of infertility in women, characterized by amenorrhea, hypoestrogenism, and elevated gonadotropin levels in women under the age of 40. POF is diagnosed on the basis of two levels of serum FSH ≥40mIU/mL taken 6 weeks apart. It affects one in 10,000 women by age 20, one in 1,000 women by age 30 and one in 100 women by age 40. Thepresent study was undertaken to evaluate biochemical and molecular instabilities on subjects with premature ovarian failure. Thirty five female subjects with premature ovarian failure and 16 healthy women without any chronic illness were involved in this study. Reproductive hormones namely leutinizing hormone (LH), follicle stimulating hormone (FSH) and estradiol were estimated in each subjects after obtaining their informed consent. Cytokinesis–block micronuclei (CBMN) assay was also carried out in the lymphocytes of the subjects to assess the somatic DNA damage. The study demonstrated that the micronuclei frequency significantly elevated in the study subjects than control subjects. Women with various risk factors such as increasing age, BMI, family history of infertility, family history of cancer, delayed menarche, endometriosis, etc. can lead to increased genetic instabilities.