Aim: To compare the quality of the PC’s prepared by three methods i.e Platelet Rich Plasma (PRP), Buffy Coat (BC) and Apheresis method as per the departmental SOP based on DGHS criteria.             Material & Methods: Four platelet concentrates prepared by each of the three methods were assessed every month for the following quality parameters – Swirling, Volume, Platelet count, WBC count and pH. Results:  48 PC of each method (Total 144) were analysed. 62.5% of PRP–PC & 58.33% of BC–PC showed Grade II swirling & 91.67% of the Apheresis–PC had grade III swirling. The mean volume of PRP–PC, BC–PC and Apheresis–PC units was 58.45 ± 13.05 ml, 60.68 ± 16.09 ml and 209.65 ±10.86 ml respectively. The mean platelet count of PRP–PC, BC–PC and Apheresis–PC units was 5.68±1.83 1010/unit, 5.13 ±1.75 x 1010 /unit and 2.72 ± 0.48 x 1011 /unit respectively. The mean WBC count of PRP– PC, BC–PC and Apheresis–PC units was 7.6 ±4.87 x 107/unit, 3.72 ± 1.63 x 107 /unit and 4.16 ± 0.71 x 106/unit & The mean pH of PRP–PC, BC–PC and Apheresis–PC units was 6.9 ± 0.32, 6.9 ± 0.33 and 7.02 ± 0.26 respectively. Conclusion: PRP–PC and BC–PC units were comparable for swirling. Apheresis–PC units showed better swirling than PRP–PC and BC–PC units. PRP–PC and BC–PC units were comparable for mean volume & Platelet count per unit; Apheresis–PC units showed better platelet counts than PRP–PC and BC–PC units. BC–PC units had less mean WBC contamination than PRP–PC units and Apheresis–PC units had least WBC contamination.