Aim: To assess the effect of Regen–D 60 Gel on the rate of healing of gingival epithelium. Materials and methods: A total of 40 sites in 10 patients (6 males and 4 females, 18 to 32 years old) with uniformly dense bands of pronounced bilateral melanin pigmentation on the facial aspect of the maxilla and mandible were selected. Sites extending from distal of the right canine to the midline and distal of the left canine to the midline underwent depigmentation. In each patient 4 quadrants were randomly allocated to one of the four treatment groups. Group I with patients who underwent depigmentation with diode laser along with application of REGEN–D  60 gel, group II with patients who underwent depigmentation with diode laser alone, group III with patients who underwent depigmentation with tetrafluoroethane along with REGEN–D 60 gel and group IV with patients who underwent depigmentation with tetrafluoroethane alone. REGEN–D 60 gel was applied randomly to treated sites following depigmentation to assess the healing. Gingival index (GI) (Silness and Loe, 1964) was recorded at baseline and 1 month after the procedure.  Healing Index (Landrey, Turnbull and Howley, 1988) was assessed at day 1, day 7, day 14 and at 1month respectively. Results: Inter group comparison of healing index scores at various time intervals showed a statistically significant difference (p˂0.0001). The change in the healing index scores between various groups from day 1 to day 14 was statistically significant (p˂0.05) Conclusion: REGEN–D 60 gel may have a beneficial effect on the rate of healing of gingival epithelium.