Klebsiella pneumoniae and staphylococcus aureus, bacterial strains are known to cause common infection in human beings. The common antibiotics used to cure the infections are Amoxicillin, Ciprofloxacin, Moxifloxacina, Cefixime, Ofloxacin and Ornidazole. The present study was aimed at understanding the efficacy of these antibiotics if exposed to UV–radiation against these bacterial infections. Both the strains i.e. Klebsiella pneumoniae and staphylococcus aureus were isolated in laboratory. They were subsequently subcultured in NAM media. The effect of the different antibiotics to be studied were exposed to UV radiations for 12 hours. The UV radiated recombinant drugs showed different levels of resistance to bacterial growth for both the strains. The zone of inhibitions, when tested for drug sensitivity, altered differently for the 6 drugs used against both the strains. For both the strains Cefixime turned out to be the most effective antibiotic, both in the unexposed form and UV exposed recombinant form.