Background: One of the principal requirements of an endodontic root canal sealer is that it should be non–cytotoxic, non–mutagenic and immunologically compatible with periapical tissues. Aim: To determine the cytotoxic potential of Chitra–CPC for the first time and compare it to a commonly used sealer AH plus and a recent dual cure resin cement Epiphany, by a colorimetric MTT assay and thus explore the possibilities of these materials in Endodontics. Materials and Methods: Cell cultures were obtained of mouse fibroblasts and subjected to the MTT colorimetric analysis with respect to exposure to the respective sealer and the percentage of viable cells was obtained for each group. Results: The toxicity of the sealer tested in this study varied from Highest to lowest in the order:  AH Plus> Epiphany>Chitra CPC. The differences were statistically significant between different sealers and at different time periods also. Conclusion: Chitra–CPC, is relatively, a biocompatible sealer as compared to AH Plus and Epiphany Sealers