Material and Method: The material for the study comprised of thirty properly embalmed adult human cadavers of either sex procured from the Department of Anatomy, Government Medical College, Amritsar. Aim: The main aim of the study was to identify the variations in location, shape and size of pyramidal lobe. Knowledge of these variations is important for planning and conducting surgical procedures on the thyroid gland. Results and Conclusion: The pyramidal lobe was present in 12(40%) cases. It was located on left side in 8(66.6%) cases, on right side in 2(16.7%) and median in 2(16.7%) cases. The pyramidal lobe was quadrangular in 9(75%) and triangular in 3(25%) cases. The mean length of the pyramidal lobe was 2.3cm ranging from minimum 1.0cm to maximum 4.6cm. Since the pyramidal lobe is a normal component of thyroid gland, of varying position and size, it should always be examined during  thyroid surgery and mandatorily removed in total and subtotal thyroidectomies.