Oral hygiene is the practice of keeping the mouth andteeth clean to prevent dental problems like, dental caries,gingivitis, periodontitis and bad breath. Tooth brushing is the mosteffective and commonly used method.Along with the brushing methods, disinfection oftoothbrush is also equally important for maintenance ofhealth of oral tissues. There is complete lack of awareness among public regarding tooth brushmaintenance. So, it is of utmost importance to educate the public about proper storage, replacement anddisinfection of tooth brushes. Hence, the purpose of this review is to explore about tooth brush contamination, its possible role in disease transmission and methods that can prevent tooth brush contamination.