Background– Genital tuberculosis (GTB) is one of the important factors that cause infertility in women. GTB is mostly asymptomatic in nature and infertility generates high suspicion for GTB in reproductive age. The object of this study was to compare the different methods to diagnose GTB in Infertile patients.

Material and method– Women patients seeking diagnosis of infertility in the OPD of Obstetrics & Gynecology department during April 2016 to March2017 were included in this study. A total of 62 endometrial samples were processed by AFB smear microscopy, L–J culture, BACTEC culture and Genexpert to diagnose the tuberculosis. Results– Out of 62 patients with infertility suspected of GTB, 26 were found to be positive either by AFB smear, L–J culture, BACTEC culture and Genexpert. Only one sample was found to be positive by all methods. Fourteen samples to be found positive with both L–J culture and BACTEC culture methods.

Conclusion– The study indicated that the old method of AFB smear is less sensitive when compared with the L–J culture and BACTEC culture. Although Genexpert have a high specificity and sensitivity but still there are limitations in the diagnosis of endometrial biopsy samples. This study suggest both liquid and solid culture have an important role in the diagnosis of genital tuberculosis.