Objective: The aim of the study was to compare the effects of different isokinetic training protocols on cardiovascular variables. Method: In the present study 60 subjects, 28 males and 32 females were selected. The subjects performed two isokinetic strength training protocols with angular velocities in reverse order – 1^st protocol was done at 60°/s, 120°/s and 180°/s  and the 2^nd protocol at 180°/s, 120°/s and 60°/s  on Biodex Isokinetic dynamometer system 4 pro. HR was measured before, during and after completion of each protocol. In recovery period BP and HR were recorded for 5 minutes after completion of each protocol. Results: The result of present study shows significant difference in HRmax and HRR when two different isokinetic training protocols are used. But there is no significant difference in SBP and DBP. Conclusion: Different orders of velocities in isokinetic training will have different effect of cardiovascular variables