The asymptomatic nature of Chlamydial genital infections coupled with the occurrence of severe sequela   in untreated patients makes the laboratory evaluation of great importance in the diagnosis of the disease. Genital chlamydial infections are the leading cause of preventable sexually transmitted infections (STIs) worldwide, with 43 million new cases in Southeast Asia. The present study was designed to determine the prevalence of genital chlamydial infection in women attending a OBGY outpatient department in a tertiary care hospital in Navi Mumbai and to determine the association of the disease with other STIs. A total of 100 female patients were enrolled for the study. Genital discharge specimens (endocervical and vaginal swab)s , together with blood/serum samples were collected from all the patients. The patients were investigated for the presence of antigen and antibody of Chlamydia trachomatis with the help of the Polymerase chain reaction (PCR) and the Enzyme Linked Immunosorbant Assay (ELISA), respectively. Investigations for aetio–pathogens of other STIs were carried out using the standard methods. Chlamydial infection was found in 3.5% to 16.7% (as per PCR) 1.8% to 33.3%(as per IgG ELISA) test. The overall incidence of other aetio–pathogens was low. Chlamydia trachomatis was found to be most commonly associated with Candida albicans and Bacterial Vaginosis..However, there was no co–infection of Chlamydia trachomatis with Neisseria gonorrhoeae,Syphilis or Vaginal Trichomoniasis  .