Spore population density, species occurrence and root colonization percentages of arbuscular mycorrhizal (AM) fungi were investigated for a period of four seasons spreading over two consecutive years on five wild ethnomedicinal plants (Adhatoda vasica, Zanthoxylum acanthopodium, Solanum nigrum, Blumea balsamifera  and Alpinia zerumbet). found in three district of Manipur, north eastern India. The spore population of AM fungi varied from a minimum of 27 spores 10 g^–1 soil in the rhizospehere of A. vasica (27) during season 3 to a maximum of 196 spores 10 g^–1 soil in  Z. acanthopodium during season 1. The AM fungal colonisation was observed highest in the roots of B. balsamifera (61.88%) during season 1 and the lowest was observed in the roots of A. zerumbet (22.67%) during season 2. A total of 20 species of AM fungi have been isolated from the rhizosphere soil of the five medicinal plants studied.  Glomus was largest genus with 13 species followed by Acaulospora with 3 species and Sclerosystis with 2 species. Only 1 species of Gigaspora was recorded in the present study. The number of AM fungi species was recorded highest in the rhizosphere soil of B.balsamifera (20), followed by S.nigrum (19) and A.zerumbet (18) while lowest of 17 AM fungi species was recorded from the rhizosphere of A. vasica and Z. acanthopodium respectively. Acaulospora  delicata was most commonly occurring AM fungus with highest (100%) occurrence in the rhizosphere of all plant species and in all seasons. Glomus macrocarpum and G. microaggregatum were other AM fungi with 95% occurrences. There was significant variation in spore density and root colonization of AM fungi between and among five plant species. The influences of season on these two parameters of AM fungi were not stable but dynamic and therefore no clear pattern was observed.  The present study recommends that cultivation practices for conservation and commercial exploitation of ethnomedicinal plants, especially the five species reported in this study may include three species of AM fungi (A. delicata, G.macrocarpum and G.microaggregatum) in the soil management activities for improving biomass production and growth performance.