Aim: The purpose of this study is to investigate the Acetylcholine in learning and memory of medical students. Acetylcholine is a very common neurotransmitter, being found in central, peripheral, autonomic and somatic nervous systems. Chemically, acetylecholine is an ester of acetate (acetic acid) and choline. Choline is a natural amine and an essential nutrient in the Vitamin B group. It is create in certain neurons by the enzyme choline acetyltransferase from the choline and acetyl–CoA. CoA is created by mitochondria and is common throughout the body. Design/Methods: This is a descriptive  Study and 90 medical students selected depending upon the university exam marks. Results: P value is < 0.001 which is significant increased serum acetylcholine concentration in good students when compared with average and poor students. Conclusions: We conclude that Medical students to improve take choline rich foods  are more prone to develop learning and memory.