Introduction– Urinary tract infections (UTI) are the second most common infections after the infections of the respiratory tract. In almost all cases of UTI, empirical antimicrobial treatment initiates before the laboratory results of urine culture are available; thus antibiotic resistance may increase in uropathogens due to frequent use of antibiotics.

Materials and Methods – All the urine  samples  of clinically suspected of UTI cases  received in the Department of  Microbiology, Government Medical College & Hospital, Nagpur wre processed by standard bacteriological procedures and the isolate was subjected to antibiotic sensitivity testing.

Results– A total number of  283 urine samples were processed,out of which  71( 25.08 %) urine samples were culture positive. The most common organism isolated was E.coli (43.66   %) . A very high level of resistance was observed among Gram negative  urinary isolates against β lactam antibiotic and cephalosporins. The only drug effective against GNR found in the present study was Amikacin and Imepenem.Gram positive cocci were sensitive to vancomycin and lenezolid.

Conclusion– Routine monitoring of susceptibility patterns is necessary. This will help in the empirical treatment of UTI to the clinicians and also for the preparation of antibiotic policy of the individual institute. This will avoid the indiscriminate use  and  ultimately prevent the further development of antimicrobial resistance.