This study was undertaken with the aim to know incidence of Ventilator–associated pneumonia (VAP) in ventilated neonates, endotracheal tube culture positivity in VAP and the outcome of babies suffering from VAP. All neonates requiring ventilator support more than 48 hours (according to CDC criteria’s for VAP) in NICU at NKPSIMS and Lata mangeshkar Hospital hingna, Nagpur from January 2015 to August 2016, were enrolled in the study, those who already had pneumonia were excluded. The incidence of VAP was calculated. Total of 100 neonates were enrolled. Results: Out of 100 ventilated neonates 18 developed VAP. Mean ventilation days= 9.3. Mean VAP/1000 ventilated days= 19.4.Most common organism is Klebsiella (43%) followed by Staphylococcus aureus (22%) and Pseudomonas(14%).Mortality in babies who had VAP is 44.4%. Conclusion–The pathogenesis of VAP involves the micro–aspiration of oropharyngeal and/or gastric secretions that have been contaminated/colonized with pathogenic organisms. Efforts to prevent VAP are focused on early extubating and preventing aspiration.  Early diagnosis and treatment help limit VAP related morbidity and mortality.