Introduction: Bloodstream infection(BSI) are an important cause of morbidity and mortality .Blood culture is use for  timely  detection and identification  of blood borne infection that is essential for appropriate antimicrobial therapy .The 7days incubation period for blood culture bottle recommended before discarding as negative for conventional method. Most of the  times result was  not useful  to change in antimicrobial therapy based on results. Comparative Study plan to evaluate the result on isolation of organism from blood culture following 3days 5days 7 days incubation protocol.Method:470 blood culture collected from all age group patients whomever clinically indicated&, processed as per plan and organism isolated by standard methods .Results: reveal 19.4 %culture positivity, 98.9 %isolates were detected after following 5days incubation protocol. Coagulase negative Staphylococci was most frequent organism. Conclusion: Study concluded that reporting 5 days incubation of blood culture bottle sufficient to say positive or negative considering its clinical implication except if ask specially for extended incubation.