In the present study to investigate the effect of Azima tetracantha on membrane bound enzymes in liver on carbon tetrachloride induced oxidative stress in rats. Decrease in activities of Na⁺/K⁺ATPase, Ca²⁺ATPase and Mg²⁺ATPase were found in CCl₄ treated animals. On treatment with different doses (100, 200 and 400mg/ Kg BW) of Azima tetracantha leaves extract (ATEE) increased in the activity as dose dependent manner. Among the various doses, 400mg/kg has potential activity than other doses. The silymarin treated rats shows restored the activities of ATPase. The results of the present study indicate that the protective role of ATEE may be related to counteraction of free radicals. Thus, ATEE stimulates the repair of mitochondrial membranes and improve mitochondrial function. These findings suggest that the protective activity of ATEE may indeed play a pivotal role in attenuating free radical damage and stabilize cellular structural integrity.