The whole human populations share the same blood group systems; although they differ in the frequencies of specific types. The incidence of ABO and Rh groups varies markedly in different races, ethnic groups and socioeconomic groups in different part of the world.The frequencies of ABO and Rh blood groups vary from one population to another and time to time in the same region,so this study was conducted to find out the frequency of different Blood Groups among the first year medical students of Assam Medical college. The study was conducted from 2006 to 2016.1806 students participated in the study. In the present study it was seen that the predominated blood group was O followed by B, A and then AB in both the sexes. The result of this study which depicts the frequency distribution of the M.B.B.S students helps in preparing  a database for the blood banks and also to create awareness as to which blood groups should be stored and given importance