Background– The assessment of health–related quality of life (QOL) is used widely as an outcome of health care system and health care interventions. Considering the health of the young professional females affects the individual growth and socio–economic growth of the country, we aimed to assess prevalence of MSDs and its impact on health–related quality of life of professional females in Delhi and NCR. Method– 300 females Subjects of age 27–35 years who were fulfilling the inclusion criteria were selected for study and out of which 270 consented to participate in the study. Kuppuswamy scale was employed to set a Socio–Economic Status (SES) baseline. To record musculoskeletal disorders, the Nordic Musculoskeletal Questionnaire was employed. Health related Quality of life was checked by SF–36 scale. Descriptive statistics and multi regress analysis were used. Result– 100% subjects were affected by musculoskeletal disorders in one or more body region. Major MSDs affected the body regions: lower back(72.9%), neck(64.4%), upper back(51.8%), knees(41.5%), shoulder(32.5%). Among eight dimensions of health related quality of life, mean score of social functioning was highest (80.74), followed by physical functioning (79.68), emotional wellbeing (74.38) and mean score of  physical role limitation (57.96) and general health (57.37) were lowest. Conclusion– The present study reports 100% prevalence of musculoskeletal disorders in professional females in one or other body region which is also affecting their quality of life.