The objective of the study was to evaluate the potential effect of ethosomal formulation of leaf extract of Mangifera indica on excision wounds in Wistar rats. The methanolic extract of Mangifera indica leaves was prepared by maceration. Three different formulations of ethosomes (100 mg, 200 mg, 300 mg) were prepared and the entrapment efficiency was 65.1%– 96.54% with average vesicle size of 926 nm. Wistar rats weighing between 180 –200 gms were taken and were divided into 5 groups (n=6). Silver nitrate ointment was used as a standard. The circular excision wound model was created on the backside of the rat neck and the percent wound area, and period of epithelisation were determined and study was conducted till the wound is healed. The % wound contraction (200 mg) was significantly reduced to 14.5 %.  The present study revealed that ethosomal gel with the test drug has significantly influenced the wound healing property.