Multidrug resistant (MDR) Pseudomonas aeruginosa is being frequently implicated in healthcare associated infections (HAIs) in the recent years. The present study was undertaken to study the antibiotic susceptibility pattern in clinical isolates of Pseudomonas aeruginosa in a tertiary care hospital. Total 126 P. aeruginosa isolates from different samples were identified by standard biochemical tests. Antibiotic susceptibility patterns of all the isolates were performed by Kirby Bauer Disc Diffusion Method (KBDDM) to five antibiotics – Ceftazidime, Ciprofloxacin, Gentamicin, Imipenem and Meropenem. By KBDDM, maximum susceptibility was observed to imipenem (79.37%), followed by meropenem (77.78%). Susceptibility to ciprofloxacin was least (31.75%). Overall 37.3% strains of P. aeruginosa were MDRs.