Tooth avulsions occur commonly due to dento–facial trauma. An ideal storage media is needed until the teeth are replanted. This study aimed to evaluate the ability of Aloe vera (AV) and Mc–carey kaufman (MK) medium as storage media in comparison with Coconut water (CW) and HBSS in maintaining the periodontal ligament (PDL) cell viability. Freshly extracted premolars were divided into 6 groups. These were stored dry for 30 mins and soaked in one of the 4 storage media i.e., HBSS, CW, Aloe vera pulp (AV) and MK medium for 45mins. Lastly they were treated with collagenase II and dispase grade II enzymes for 30 mins. The number of viable PDL cells were counted with the help of hemocytometer. CW and AV showed significantly more viable cells in comparison to HBSS and MK (p < .05). Thus it was concluded that AV had the potential of maintaining the PDL cell viability.