CONTEXT:  Campylobacter is an undected cause of diarrhoea especially under 5 years of age in most of the countries . Isolation of the organism is difficult,expensive and cumbersome. AIMS; Our objective of this study was to isolate this pathogen from the stool specimens on routinely available blood containing laboratory media using the candle jar for creating the microaerophilic  atmosphere  in our setup. SETTINGS AND DESIGNS: A descriptive study.  MATERIALS AND METHODS: A total of 100 stool samples were inoculated onto selective and non–selective media with and without filtration using 0.45 um membrane. The inoculated media were simultaneously  incubated in microaerophilic conditions using the Candle jar at temperatures 37^Oc  and 42⁰C. The culture isolates were confirmed by standard phenotypic tests. A simplex polymerase chain reaction (PCR) targeting  the 16S ribosomal deoxyribonucleic acid of Campylobacter was performed on the deoxyribonucleic acid (DNA)  of the culture isolates as well as on the DNA extracted from the stool filtrates. STATISTICAL ANALYSIS: Data was expressed as proportion. RESULTS: Campylobacter could be isolated in 10 out of 100 stool samples . Furthermore, we did not find any difference between the isolation using the selective and blood containing  media as well as the different incubation temperatures.  All the ten were confirmed phenotypically  and genotypically  to be Campylobacter jejuni. The PCR results corroborated with that of the culture.CONCLUSIONS: Isolation by culture was as sensitive as that of the PCR.