Background: Semen analysis is the index of efficiency of spermatogenesis. In spite of being mainstay of male evaluation semen analysis is the most neglected test in terms of patient preparation, collection of sample and even examination. The aim of the present study is to perform semen analysis according to WHO criteria and to use an objective scoring system to grade the semen quality. With the above aim in mind, 158 infertile males and 25 controls (with proven fertility) were included in this study. These were referred to the pathology department from obstetric and gynaecology OPD. Material and Method: Samples of semen analysis were collected in the department of pathology in sterile plastic containers by masturbation. A thorough physical examination was done and history was taken. Routine semen analysis was done within one hour of collection. Hypoosmotic Swelling Test was done in all the cases (wherever applicable) and Mixed Antiglobulin Reaction was done in the patients with deranged HOS test. Result: Semen analysis therefore forms the basis of evaluation of male infertility and secondly with help of scoring system we can segregate our patients for further benefits by assisted reproduction techniques accordinglystandards and there is great amount of overlapping between infertile males and those with proven evidence of fertility. Therefore WHO itself suggests each laboratory to establish its own reference range.(4)