Oxidative stress can be occurred by chemical compound such as CCl₄, the increasing of SGOT, as the factor of liver malfunction and increase the malondialdehyde levels as the oxidative stress’ symptom. Antioxidants play a great role for the health of human’s body because it can inhibit and neutralize oxidation reaction which involved free radicals. Dewandaru fruit (Eugina uniflora L.) has a bioactive compound that is flavonoid sequiterpen antioxidants, vitamin C and anthocyanin. This research aims to test the activity of hepatoprotective effect from Dewandaru fruit extract and the decreasing of oxidative stress through the malondialdehyde levels. Research method post test randomized post test only control group design. The research result is giving ethanol extract 50 mg/kg dosage, 100 mg/kg dosage, 200 mg/kg dosage had decreased SGOT, SGPT, and MDA significantly (p < 0,05) to the CCl₄ injected mouse. The highest level of reduction occurred in 200 mg/kg b.w dosage.